SAFE RAW MATERIALS IN EXPERIENCE ON LABORATORY ANIMALS

Abstract

Initially, raw materials of animal and vegetable origin, as well as minced meat semi-finished products obtained from them, were examined for safety indicators according to the requirements of SanPiN 2.3.2.1078-01. Since ground beef in dry form is preferred as an animal protein in the diet of laboratory animals - white rats of the Wistar line, we chose the meat of animals of the Hereford breed from the Novosibirsk region. Sea buckthorn fruits of the Chechek variety of Altai selection are the best source of biologically active substances in terms of the content of pectins, oilseed sugars compared to local varieties, so this particular variety was chosen for research as a detoxifier. The preventive effectiveness of the developed minced meat semi-finished product was tested when fed to laboratory animals. After conducting an in vivo experiment, the concentration of cadmium and lead was determined in their organs and tissues. In General, the concentration of cadmium ions decreased by 2-3. 6 times (P<0.01), and lead ions - by 1.97-3.5 times (P<0.01). The conducted research proved the safety of animal and vegetable raw materials, the resulting minced meat semi-finished product and the effectiveness of using sea buckthorn semi-finished product to reduce the accumulation of cadmium and lead in the organs and tissues of laboratory animals.