Application of molecular genetic methods for identification of genetically modified soy beans in order to improve the quality and safety of products
https://doi.org/10.31677/2311-0651-2025-48-2-35-41
Abstract
The article presents the results of a study of methods for the identification of genetically modified soybeans using polymerase chain reaction (PCR). The aim of the study was to control the quality of vegetable raw materials - soybeans by PCR. In the course of the work, modern laboratory devices (“Real-time CFX Connect”, “Rotor-Gene 6000”) and specialized reagents “Vet-Factor” were used. The technique included several stages: sampling, DNA isolation, reaction and analysis of the results. It has been experimentally established that the nutrient content in the studied soybean samples has differences. The genetically modified soybeans showed a decrease in protein, fat, fiber, and phosphorus levels by 3.0–12.0% compared with native soybeans. At the same time, the calcium content in GM soybeans is 11% higher. Based on the data obtained, the authors recommend using native soybeans for food production, and GM soybeans mainly for feed production. The conducted studies have confirmed the effectiveness of using the PCR method for the identification of GMOs in plant raw materials. The data obtained can be used to improve the quality control system of agricultural products and ensure food security.
About the Authors
Sh. A. Pfeiffer
Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
Russian Federation
Russian Federation
PhD student
F. Ya. Rudik
Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
Russian Federation
Russian Federation
Doctor of Technical Sciences, Professor
O. S. Fomenko
Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
Russian Federation
Russian Federation
Candidate of Technical Sciences, Associate Professor
O. M. Buttaev
Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov
Russian Federation
Russian Federation
Candidate of Technical Sciences, Associate Professor
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Review
For citations:
Pfeiffer Sh.A., Rudik F.Ya., Fomenko O.S., Buttaev O.M. Application of molecular genetic methods for identification of genetically modified soy beans in order to improve the quality and safety of products. Innovations and Food Safety. 2025;(2):35-41. (In Russ.) https://doi.org/10.31677/2311-0651-2025-48-2-35-41
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