EPIZOOTOLOGICAL AND PHYLOGENETIC EVALUATION OF THE BOVINE LEUKEMIA VIRUS IN RUSSIAN FEDERATION

The genetic characterization of BLV is an important task in scientific research in many countries of the world. According to the sequenced gene region - env BLV isolates allocated in different geographical locations of the world, up to 10 different genetic groups of the virus were identified and classified. Acknowledgments: The research was carried out at the expense of the Russian Science Foundation grant (project No. 17-76-10051). As part of the research, we monitored the epizootic situation of the BLV in Russia. Groups of animals (n=50) Holstein-Frisian (imported breed) and Russian Black Pied (local breed) were selected, belonging to agricultural organizations of the Tyumen region. Serological screening methods (ELISA, AGID) were used in cattle to identify infected animals. Immunological evaluation of animals in all test groups is given. A primary A nested-PCR study was performed, which resulted in a fragment of the env 444 bp gene in the studied samples. RFLP analysis of this fragment allowed to establish that in 94 % of the samples there was a «Belgian type» of the leukemia virus, in 4 % of samples - «Australian» and in 2 % - a «mixed type». All samples were sent for NGS sequencing. By phylogenetic evaluation of the BLV genome env site in the isolated samples and the immunological evaluation of the infected animals, new data will be obtained that will allow updating information on the genetic groups of the BLV in the territory of the Russian Federation.

Bovine leukemia virus, cattle, phylogenetic classification, epizootic situation, serological screening, polymerase chain reaction, genetic polymorphism, epizootic monitoring

1. OIE World animal health infromation database-version: 1.4. Paris France: World organisation for animal Health. - 2009.

2. OIE World Organization for Animal Health. Enzootic Bovine Leukosis. World Anim Heal Inf Database, Dis information, List Ctries by Sanit Situat. Available [Электрон. ресурс]. - 2017. - Режим доступа: http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/statuslist.

3. LaDronka R. Impact of bovine leukemia virus on herd level production indicators on U.S. dairy farms// 97th Annual Meeting of the Conference of Research Workers in Animal Diseases. - Chicago, IL, 2016.

4. Molecular characterization of bovine leukemia virus from Moldovan dairy cattle/ A. Pluta, M. Rola-Łuszczak, P. Kubis´ [et al.] // Arch. Virol. - 2018. - P. 1563-1576.

5. Polat M., Takeshima S., Aida Y. Epidemiology and genetic diversity of bovine leukemia virus// Virology Journal. - 2017. - Vol. 14. - P. 209. - DOI: 10.1186/s12985-017-0876-4.

6. Эпизоотическая ситуация по социально значимым и особо опасным болезням животных в Российской Федерации за 2016 год [Электрон. ресурс] / В. Н. Шевкопляс, В. Н. Боровой, Ю. И. Барсуков, С. А. Коломыцев. - Режим доступа: https://www.tsenovik.ru/bizness/articles/mvet/epizooticheskaya-situatsiya-po-sotsialno-znachimym-i-osobo-opasnym-boleznyam-zhivotnykh-v-rossiyskoy-1/.

7. Гулюкин М. И., Донник И. М., Татарчук А. Т. Методологическая система оздоровительных мероприятий при лейкозе крупного рогатого скота. - Екатеринбург: Урал. изд-во, 2007. - 224 с.

8. Методы лабораторной диагностики лейкоза: учеб. пособие/ И. М. Донник, И. А. Шкуратова, А. С. Кривоногова, М. В. Петропавловский [и др.]. - Екатеринбург: Урал. гос. агр. ун-т, 2015. - 48 с.

9. Features of the regional structure of the blv provirus/ N. V. Bateneva, P. N. Smirnov, S. H. Vyshegurov [et al.] // Research Journal of Pharmaceutical, Biological and Chemical Sciences. - 2016. - Т. 7, N 4. - P. 2025-2032.

10. Батенёва Н. В. Особенности течения лейкозного процесса у носителей 4-го и 7-го генотипов BLV // Инновации и продовольственная безопасность. - 2015. - № 4 (10). - С. 5-8.

11. Molecular analysis of a 444 bp fragment of the bovine leukaemia virus gp51 env gene reveals a high frequency of non-silent point mutations and suggests the presence of two subgroups of BLV in Chile/ R. Felmer, G. Mun˜oz, J. Zu´n˜iga, M. Recabal // Vet. Microbiol. - 2005. - Vol. 108 (1-2) - P. 39-47.

12. Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle/ E. Lee, E. J. Kim, J. Ratthanophart [et al.] // Infect Genet Evol. - 2016. - Vol. 41. - P. 245-254.

13. Sequencing and phylogenetic analysis of the gp51 gene from Korean bovine leukemia virus isolates/ E. Lee, E. J. Kim, H. K. Joung [et al.] // Virol. J. - 2015. - Vol. 12. - P. 64. - DOI: 10.1186/s12985-015-0286-4.

14. Detection and molecular characterization of bovine leukemia virus in Philippine cattle /M. Polat, A. Ohno, S. N. Takeshima [et al.] // Arch. Virol. - 2016. - Vol. 120. - P. 285-296. - DOI: 10.1007/s00705-014-2280-3.

15. Detection and molecular characterization of bovine leukemia virus in Philippine cattle/ M. Polat, A. Ohno, S. N. Takeshima, J. Kim, M. Kikuya, Y. Matsumoto, C. N. Mingala, M. Onuma, Y. Aida // Arch. Virol. - 2015. - Vol. - 160. - P. 285-296.

16. A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis/ M. Polat, S. N. Takeshima, K. Hosomichi, [et al.] // Retrovirology. - 2016. Vol. -13 P. 4.

17. The molecular epidemiological study of bovine leukemia virus infection in Myanmar cattle/ M. Polat, H. H. Moe, T. Shimogiri [et al.] // Arch. Virol. - 2016. - Vol. 1. - P. 293.

18. The molecular characterization of bovine leukemia virus 1574 A. Pluta et al. isolates from Eastern Europe and Siberia and its impact on phylogeny/ M. Rola-Łuszczak, A. Pluta, M. Olech [et al.] // PLoS One. - 2018. - DOI: 10.1371/journal.pone.0058705.

19. Molecular epidemiology and characterization of bovine leukemia virus in domestic yaks (Bos grunniens) on the Qinghai-Tibet Plateau, China/ M. Wang [et al.] //Arch. Virol. - 2018. - Vol. 163 (3). - P. - 659-670.

20. First molecular characterization of bovine leukemia virus infections in the Caribbean/ Y. Yang, P. J. Kelly, J. Bai, R. Zhang, C. Wang // PLoS One. - 2016.