IDENTIFICATION OF EPIDEMICALLY-SIGNIFICANT SALMONELLA SEROTYPE USING IMMUNOFLUORESCENCE AGGLUTINATION REACTION (IRA)

Abstract

In the Russian Federation, the presence of all serotypes of Salmonella in products is unacceptable. However, the existing ELISA tests are applicable for seromonitoring for the presence of antibodies only to Salmonella of several serotypes. In the article, the authors proposed a system based on the agglutination reaction with a fluorescently labeled antigen - IRA. The purpose of the study is to demonstrate the use of IRA in the example of an analysis of the epizootic situation at one of the poultry farms in Russia and to show an example of the interpretation of the results of serological studies to control bacterial infections in broiler chickens. The peculiarity of the new modification is that in ultraviolet rays the negative reaction of the “button” glows in the form of a dot. The authors studied sera from a herd of broiler chickens affected by Salmonellosis and found antibodies to Salmonella of two serotypes, Infantis and Hamburg. The analysis of seroprevalence to these two Salmonella serotypes made it possible to assess the greater epizootic significance for Hamburg serotype Salmonella. The use of the IRA, incl. based on antigens from bacterial cultures isolated from poultry products, and pathological material, allows for serological diagnosis of bacterial infections of birds concerning the entire variety of bacteria that can be found in a poultry farm, unlike ELISA systems.