THE FREQUENCY OF INDICATION AND ANTIBIOTIC SUSCEPTIBILITY OF E. COLI ISOLATES. COLI O157:H7 ISOLATED FROM VARIOUS SUBSTANCE
https://doi.org/10.31677/2311-0651-2022-35-1-49-55
Abstract
The authors studied the indication and antibiotic sensitivity of E. coli O157:H7 isolates isolated from soil, meat samples and objects subjected to veterinary surveillance. In a study on isolation of Enterohaemorrhagic E. coli from various environmental media 6602 samples were examined. The highest number of E. coli O157:H7 was isolated from soil in 10 % and meat in 8.7 % of cases. E. coli O157:H7 was isolated in 2.6% of cases from wipes of objects subjected to veterinary surveillance. This microorganism was not detected in feed of plant origin. Antibacterial sensitivity studies of isolated E. coli O157:H7 isolates revealed 100% sensitivity to cefotaxime, cefepime, imipenem, ciprofloxacin, ceftriaxone. The authors observed an 80-90% sensitivity of isolated strains to norfloxacin, cefoxitin, gentamicin and ceftazidime. Studies also showed complete resistance of E. coli O157:H7 isolates to tetracycline in 80% of microorganisms. Of these, 2 cultures were isolated from soil and 6 cultures from meat from forced slaughter.
About the Authors
O. N. LarinaRussian Federation
Postgraduate Student
I. S. Onishchenko
Russian Federation
Ph.D. in Veterinary Sciences
M. A. Timofeeva
Russian Federation
Postgraduate Student
N. A. Shkil
Russian Federation
Doctor of Veterinary Sciences, Professor
References
1. Feiner R., Argov T., Rabinovich L. A new perspective on lysogeny: pro-phages as active regulatory switches of bacteria, Nature Reviews Microbiology, 2015, No. 13, pp. 641-650.
2. Shiruma N.J., Pilhofer M., Weiss G.L. Marine tubeformmetamophosis in-duced by arrays of bacterial phage tail-like structures, Science, 2014, No. 343, pp. 529-533.
3. Kashin A.S., Zazdravnyh M.I., Shkil’ N.A., Kolibakterioz telyat v sovremennyh ekologicheskih usloviyah Sibiri (Colibacteriosis of calves in modern ecological conditions of Siberia), Method. Recommendations, Barnaul: AzBuka, 2003, 79 p.
4. Kaftyreva L.A., Makarova M.A., Konovalova T.A., Zhurnal mikrobiologii, epidemio-logii i immunologii, 2013, No. 5, pp. 100–104. (In Russ.)
5. Ryapis L.A., Filatov N.N., Svetoch E.A., Mikrobiologiya, 2004, No. 12, pp. 7–11. (In Russ.)
6. Padhye N.V., Doyle M.P. Escherichia coli O157:H7 epidemiology, path-ogenesis and methods for detection in food, J. Food Protect, 1992, No. 55, pp. 555-565.
7. Jaros P., Prattley D., Marshall J.C. Geographic divergence of bovine and human shiga toxin–producing Escherichia coli O157:H7 genotypes, new Zealand, Emerging Infectious Diseases, 2014, Vol. 20, No. 12, pp. 1980-1989.
8. Wong C.S., Jelacic S., Habeeb R.L. [et al.] The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections, N. Engl. J. Med, 2000, Vol. 342, pp. 1930-1936.
9. Stepanshin Yu.G., Svetoch E.A., Eruslanov B.V., Veterinariya, 2005, No. 7, pp. 17–22. (In Russ.)
10. Onishchenko I.S., Shkil’ N.A., Koptev V.Yu., Aktual’nye voprosy veterinarnoj mediciny Sibiri, Krasnoobsk, 2010, pp. 75–83. (In Russ.)
11. Sargeant J.M., Oberst R.D., Phebus R.K. Results of a longitudinal study of the prevalence of Escherichia coli O157:H7 on cow-calf farms, Am. J. Vet. Res, 2000, Vol. 61, No. 11, pp. 1375-1379.
12. Pushkareva V.I., Litvin V.Yu., Ermolaeva S.A., Epidemiologiya i vakci-noprofilaktika, 2012, No. 2 (63), pp. 10–20. (In Russ.)
13. Jayaraman D., Valdés-López O. Response of medicago truncatula seed-lings to colonization by salmonella enterica and escherichia coli O157:H7, PLoS ONE, 2014, Vol. 9, No. 2, pp. 1-12.
14. Sidorenko S.V. Genodiagnostika infekcionnyh boleznej (Genodiagnostics of infectious diseases), Proceedings of the Russian Scientific and Practical Conference, Novosibirsk, 25-27 October’s, Novosibirsk, 2005, Vol. II, pp. 129–130. (In Russ.)
15. Pirozhkov M.K., Strel’chenko S.A. Profilaktika, diagnostika i lechenie infekcionnyh boleznej, obshchih dlya lyudej i zhivotnyh (Prevention, diagnosis and treatment of infectious diseases common to humans and animals), Proceedings of the International Scientific Conference, 27-23 June, 2006, Ul’yanovsk, 2006, pp. 119–122. (In Russ.)
Review
For citations:
Larina O.N., Onishchenko I.S., Timofeeva M.A., Shkil N.A. THE FREQUENCY OF INDICATION AND ANTIBIOTIC SUSCEPTIBILITY OF E. COLI ISOLATES. COLI O157:H7 ISOLATED FROM VARIOUS SUBSTANCE. Innovations and Food Safety. 2022;(1):49-55. (In Russ.) https://doi.org/10.31677/2311-0651-2022-35-1-49-55