BIOMEDICAL POTENTIAL OF THE SPORE-RECOMBINANT STRAIN BACILLUS SUBTILIS 2335/105 IN THE COMPOSITION OF SUBALIN®, AS A LIVING THERAPEUTIC SYSTEM FOR THE DELIVERY OF ALPHA-INTERFERON IN VIRAL LEUKEMIA: ASSESSMENT OF THE EFFECT ON IMMUNE CELLS AND ORGANS OF MICE IN THE EXPERIMENT OF VIRAL LEUKEMIA
https://doi.org/10.31677/2311-0651-2019-24-2-85-93
Abstract
<p>To maintain a healthy immune system in animals, especially under conditions unfavorable for viral leukemia, a promising delivery system of biologically active molecules is considered – Subalin, a live delivery system. As a result of research, it has been established that with intraperitoneal and intravenous administration to animals, Subalin is harmless to animals, even at doses many times higher than the therapeutic one. With the introduction of the drug Subalin in animals stimulated humoral and cellular immunity, as evidenced by morphofunctional reorganization of the lymphoid system (spleen, lymph nodes). With the simultaneous administration of Subaline with a virus-containing suspension, subtility therapy had a stabilizing effect on the macroorganism, restraining the development of viral carcinogenesis.</p>
About the Authors
Ya. L. Rusakova
National Medical Research Center. Academician E.N. Meshalkin
Russian Federation
Junior Researcher
Russian Federation
Junior Researcher
O. V. Kazakov
Research Institute of Clinical and Experimental Lymphology
Russian Federation
Candidate of Biological Sciences
Russian Federation
Candidate of Biological Sciences
V. A. Belyavskaya
State Scientific Center of Virology and Biotechnology
Russian Federation
Doctor of Biological Sciences, Professor
Russian Federation
Doctor of Biological Sciences, Professor
References
1. Infectious agents and cancer: criteria for a causal relation. / J. S. Pagano, M. Blaser, M. A. Buendia [et al.] // Semin Cancer Biol. - 2004. - Vol. 14.P. 453–471.
2. W. A. Tompkins. Immunomodulation and the therapeutic effects of the oral interferon -α: the mechanism of action // J. Interf. Cytok. Res. - 1997.Vol. 19. - p. 817–828.
3. Chen H., Ullah J., Jia J. Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis // J. Mol. Microbiol. Biotechnol. - 2017. - Vol. 27. - p. 159–167.
4. Patent No. 1839459. Bacillus subtilis bacterial strain, which has antiviral and antibacterial activity / V.V. Smirnov, V.A. Belyavskaya, A.A. Il’ichev [and others]. - 1994.
5. Biological effects of interferon produced by the recombinant bacteria of the probiotic drug Subalin / V. A. Belyavskaya, N. V. Cherdyntseva, V. M. Bondarenko, N. V. Litvyakov // Journal of Microbiology. - 2003. - № 6. - pp. 102–109.
6. The role of macrophages in the implementation of the antiblastomic action of recombinant probiotic Subalin / N. V. Litvyakov, N. V. Cherdyntseva, V. A. Belyavskaya [and others] // Oncology issues. - 2001. - V. 47, No. 1. - P. 86–89.
7. Experimental evaluation of the biosafety of genetically engineered bacteria on a model of the strain of Bacillus subtilis producing interferon / V. A. Belyavskaya, TA A. Kashperova, V. M. Bondarenko [and others]. // Journal. microbiology, epidemiology, and immunobiology. - 2001. - №2. - P. 16–20.
8. Antiviral activity of probiotics from bacilli / N.V. Chudnovskaya, I. B. Sorokulova, V. A. Belyavskaya, B. B. Smirnov // Dokl. Academy of Sciences of Ukraine. 1995. - No. 2. - P. 124–126.
9. The influence of recombinant probiotic Subalin on the functional activity of immunocompetent cells / N. V. Cherdyntseva, N. V. Litvyakov, V. A. Belyavskaya, E. S. Smolyaninov // Byul. experiment. biology. - 1999. - No. 127 (Annex 1). - p. 67–70.
10. Adjuvant properties of recombinant probiotic Subalin producing interferon / V. A. Belyavskaya, G. M. Ignatiev, N. V. Litvyakov, N. V. Cherdyntseva // Zhurn. microbiology, epidemiology, immunobiology. - 2001. - № 6. - p. 77–82.
11. T-cell leukemia virus / Y. Aida, H. Murakami, M. Takahashi [et al.], Mechanisms of pathogenesis induced by bovine leukemia, // Microbiol. - 2013. - Vol. 4. - P. 328.
12. Umar Mustafa Halit. Aspects of the pathogenesis of the rauscher murine leukemia virus infection. - Bronder-Offset B.V. - Rotterdam, 1977. - 126 p.
13. Rocchietta J. The use of Bacillus subtilis in the treatment of diseases // Mi nerva Med. - 1969. - Vol. 60. - P.117–123.
14. Rusakova Ya. L., Mager S.N., Khramtsov V.V. Effect of stem cells on the immune response in BALB / c mice infected with Rauscher leukemia virus and tolerability of Subalin and Silverol preparations // Vestn. NSAU. - 2012. - №2 (23). - p. 75–79.
15. Rusakova Ya. L., Mager S.N., Khramtsov V.V. Impact of the “Subalin” preparation on the hematological parameters of BALB / c mice infected with Rauscher leukemia virus // Vestn. Buryat. state S.-H. Acad. them. V.R. Filippova. - 2015. - №3 (40). - pp. 57–62.
16. TU 9383004–00479379–98 series 020910
17. Manual on the use of Subalin in veterinary medicine №13-5-2 / 1423
18. RD 42–28–8–89. Preclinical testing of new medical immunobiological preparations. The main provisions / M-USSR Health. - M., 1989. - 31c. - Enter 04/10/89
19. Rusakova Ya. L., Mager S.N., Khramtsov V.V. Impact of the Rauscher leukemia virus on hematological indices and the morphology of the lymph nodes of experimental BALB / c mice // Vestn. NSAU. - 2014. - №3 (32). - pp. 104–109.
20. Volkova OV, Eletsky B.K. Fundamentals of histology and histological techniques. - M .: Medicine, 1971. - p. 242–253.
21. Romeus B. Microscopic technique. - M .: Inostr. lit., 1954. - 718 p.
22. Stefanov S. B. Visual classification in quantitative comparison of images // Archive of Anatomy, Histology and Embryology. - 1985. - V. 88, no. 2. - pp. 78–83.
23. International histological nomenclature / under total. ed. Yu. I. Afanasyev. - 5th ed. - M .: Medicine, 1987. - p. 128.
24. Abramov M. G. Hematological Atlas. - M .: Medicine, 1985. - 344 p.
25. Katinas G. S., Lyashko O. G., Bazhenova I. A. Dynamics of the number of lymphoid cells in the paracortical zone of lymph nodes in C57B1 mice // Temporal and spatial organization of tissues. - L .: Izd-vo I LMI, 1981. - P. 47–54.
Review
For citations:
Rusakova Ya.L., Kazakov O.V., Belyavskaya V.A. BIOMEDICAL POTENTIAL OF THE SPORE-RECOMBINANT STRAIN BACILLUS SUBTILIS 2335/105 IN THE COMPOSITION OF SUBALIN®, AS A LIVING THERAPEUTIC SYSTEM FOR THE DELIVERY OF ALPHA-INTERFERON IN VIRAL LEUKEMIA: ASSESSMENT OF THE EFFECT ON IMMUNE CELLS AND ORGANS OF MICE IN THE EXPERIMENT OF VIRAL LEUKEMIA. Innovations and Food Safety. 2019;(2):85-93. (In Russ.) https://doi.org/10.31677/2311-0651-2019-24-2-85-93
Views:
333
Email this article 